Association of Vitamin E Level in Follicular Fluid with Morphology of Oocyte and Quality of Embryo in IVF Patients, Alzahra Hospital Rasht

Abstract:
Background And Objective
As a result of globally increasing trend in infertility, taking advantage of reproductive technology to deal with this issue is recommended. Taking the well-founded claim about Vitamin E as a powerful antioxidant with a protective role in the body, this study was instigated to find the relationship between levels of vitamin E in Follicular fluid and morphology of oocyte along with embryo quality in IVF patients.
Materials And Methods
This cross-sectional analytical study was carried out on oocytes and embryos obtained from 50 women with IVF admitted to Al Zahra Hospital of Rasht city. Patients underwent a similar ovulation stimulation protocol and then ten thousand units of hCG injected. After 36 hours, they underwent Follicle suction and mature oocytes were isolated for fertilization. Vitamin E level was measured and consequently the morphology of oocytes and the quality of embryo were evaluated by means of inverted optical microscopy.
Results
Of 50 treated IVF women, 583 oocytes and 275 embryos were examined. No significant relationship was observed between age, BMI, duration or cause of infertility and Vitamin E (P>0/05). At 0.5-1 mg/dl level of vitamin E, there were higher MII oocytes in comparison with other levels (p= 0.014). Despite the mean number of 2PN embryos at this level of vitamin E was more than other levels, this difference was not statistically significant (P=0.872).
Conclusion
The results prove the evidence that at 0.5-1mg/dl level of Vitamin E, oocyte morphology and embryo quality were significantly improved.
Language:
Persian
Published:
Journal of Advances in Medical and Biomedical Research, Volume:24 Issue: 102, 2016
Pages:
21 to 31
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