Antibiotic resistance and distribution of beta-lactamase resistance genes in Escherichia coli strains isolated from urinary tract infection in women and children in the city Farsan

Urinary tract infection is the most common infections in women and children that E.coli is considered the main cause of infection. Resistance against antibiotics used in the treatment procedure is an important problem. This study aimed to determine the prevalence of antibiotic resistance and related genes of E.coli in outpatients referred to clinics of Farsan. A cross - sectional study of 95 patients, women, and children with urinary tract infection was collected in 1392 in the clinics of farsan city. Samples of bacteria grown on Blood Agar and Eosin Methylene Blue Agar(EMB) then using antibiogram Kirby-bauer method and the zone of growth inhibition were examined. The Polymerase Chain Reaction(PCR) was usesd to determine the prevalence of resistance genes CTX, CTX-M, TEM and B-SHV respectively. Based on the results of antimicrobial resistance test, respectively the highest resistance to ampicillin (78/95%), trimethoprim - sulfamethoxazole (10/62%), nitrofurantoin (10/42%) and the most sensitive to ciprofloxacin (31/46%) and ceftriaxone (21/44%) were reported. The PCR results showed CTX was the highest and SHV-B was the lowest prevalence between resistance genes. The results showed the prevalence of Escherichia coli producing beta-lactamase resistance genes are on the rise. It is also better not to use ampicillin, trimethoprim - sulfamethoxazole and nitrofurantoin in the primary treatment of urinary tract infections.