The role of ISCOMATRIX bilayer composition to induce a cell mediated immunity and protection against leishmaniasis in BALB/c mice
Author(s):
Abstract:
Objective(s)
Development of new generation of vaccines against leishmaniasis is possible because long-term protection is usually seen after recovery from cutaneous leishmaniasis. ISCOMATRIX is particulate antigen delivery system composed of antigen, cholesterol, phospholipid and saponin. In this study, the role of ISCOMATRIX bilayer composition made by different phase transition temperature (Tc) to induce a type of immune response and protection against leishmaniasis was assessed.Materials And Methods
ISCOMATRIX formulations with different bilayer compositions consisting of EPC (Tc Results
Although the groups of mice immunized with ISCOMATRIX DMPC or ISCOMATRIX DSPC showed the smallest footpad swelling and least parasite burden compared with the buffer group, the difference was not significant. Moreover, the highest level of IFN- γ and IL-4 was observed in the splenocytes of mice immunized with ISCOMATRIX DMPC or ISCOMATRIX DSPC, respectively. After challenge, the mice immunized with ISCOMATRIX DSPC showed the highest elevation of IgG, IgG1 and IgG2a antibodies (PConclusion
Our results showed that the adjuvanticity of prepared ISCOMATRIX doesnt influence with different phospholipids at least in our mice model. Keywords:
Language:
English
Published:
Iranian Journal of Basic Medical Sciences, Volume:19 Issue: 2, Feb 2016
Pages:
178 to 186
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