Effect of 8 weeks resistance training on plasma irisin protein level and muscle FNDC5 and adipose tissue UCP1 genes expression in male rats

The purpose of this study was to determine the effect of 8weeks resistance training on plasma irisin protein level and expression of soleus muscle FNDC5 and subcutaneous adipose tissue UCP1 genes in male rats. 16 Sprague dolly male rats (w=211.5± 22.2 gr, 8 weeks) divided in 2(n=8) groups (8weeks resistance training and control group) randomly. Training group exercised 3 times per week for 8 weeks on a special ladder (1.2 meters height) and carried a weight of 50% of body weight, which was closed with their tails, began their training and reached to 200% of the body weight of rats in the final week. 3 sets of 5 reps with 3 minutes rest between training sessions and one minute between repetitions was done. ELISA method for measuring plasma irisin levels and real time PCR method was used for the relative expression of mRNA genes UCP1 and fndc5. Independent t test was used to compare group differences (α=0.05). Results showed that irisin protein levels after 8 weeks of resistance training significantly increased (p