The effect of different concentration of zinc nano complex on bull semen quality and pregnancy outcomes
Author(s):
Abstract:
This study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and percentage of pregnancy after freeze-thawing process. Ejaculates were collected from four Holstein bulls, twice a week during four weeks. semen samples diluted with Bioxcell extender containing 0 (Control), 10-6 nM, 10-5 nM,10-4 nM, 10-3 nM, and 10-2 nM of zinc nano-complex. then, were freezed and thawed after 72 hours. Motility of sperm was analyzed with computer assisted sperm analysis. the highest and lowest concentrations of zinc nano-complex along with control group were analyzed for the DNA fragmentation, malondialdehyde (MDA) concentration, mitochondrial activity, and pregnancy outcomes. The proportion of the total and progressive motile spermatozoa and the percentage of spermatozoa with fragmented DNA was not different among the groups. . However, the groups containing 10-2 nM and 10-6 nM zinc nano-complex had a lower level of MDA concentration and higher activity of mitochondria. Moreover, the pregnancy outcome was not affected by the treatment with zinc nano-complex. In conclusion, our data revealed that supplementation of bull semen Bioxcell extender with zinc nano-complex can decrease the level of MDA and improve mitochondrial activity, although such an improvement was not reflected in results of fertility rates.
Keywords:
bull sperm , DNA fragmentation , Mitochondrial , activity , MDA , Nano , particle
Language:
Persian
Published:
Journal of Animal Productions, Volume:18 Issue: 1, 2016
Pages:
173 to 181
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