Prevalence study of Eimeria species in broilers and layer chickens pathology in Tehran and Alborz provinces

Brucellosis is a zoonotic disease that spread from infected animals to humans. Six classic species of Brucella include
B. abortus, B. melitensis, B. suis, B. canis, B. neotomae and B. ovis. there. Four new species of Brucella including B. pinnipediae, B. inopinata B. cetaceae and B. maris have been reported in recent years. There are several methods to diagnosis of brucellosis. Some of these methods contain agglutination tests, such as Wright - Rose Bengal and ring test. The expiry date of these products are considered for one year in Razi Institute. Since the expiry date of foreign similar products is greater than one year also OIE and WHO guidelines are not mandatory on the one-year expiration of these products. So this project is to investigate the possibility of increasing the expiration date of the products for longer storing time and provides easy access to consumers. For this project, the antigens were sampled and the following two methods were used: 1- Real Time Stability Study. The standard method for determining the product expiry date is Real Time Stability Study . In this method, the sample antigens were tested at production time for 3 years in one-year periodical durations. 2- Accelerated Stability Study. The results showed that the physical and chemical characteristics including packed cell volume, sterility, phenol, pH, agglutination test with standard serum of Rose Bengal, Wright and ring test antigens did not show marked changes rather than specifications feature at production time and standard sample. Therefore, the results indicated that the expiry date of Rose Bengal, sero agglutination Wright and Milk Ring antigens can be considered for 3 years.