Molecular identification of escherichia coli pathotypes EAEC and EPEC strains isolated from dairy cows with mastitis by Multiplex- PCR and determination of antibiotic resistance by disk diffusion method and E-test

E.coli as normal intestinal tract flora of animals and humans are harmless bacteria.Although most strain of E.coli are not pathogenic, but some strains can cause a variety of enteritidis and non-enteritidis diseases. Antibiotic resistance microorganisms are important in treatment of infectious diseases. The aim of this study was to investigate the frequency of genes pathotypes EAEC, EPEC and antibiotic resistance Escherichia coli isolates from animal specimens. Collected 50 samples had undergone various biochemical and microbiological tests to identification or confirmation of microorganisms. Then antibiotic susceptibility tests were performed by disk diffusion method according to CLSI guidelines. E-test was performed with different antibiotics groups. Multiplex PCR assay was used to identify genes pathotypes. All of the clinically isolated E.coli was susceptible to erythromycin (100%), resistance to ampicillin (93%), sensitive to amikacin (100%) and susceptible to nitrofurantoin (96%). Many strains were multidrug resistance. The results of 50 samples of cattle milk Multiplex PCR haveshown four samples (8%) carried gene bfPA (pathotypes EPEC). The results of the M-PCR in this study were inconsistent with the results of other study has done in different countries. This Inconsistency may raise due the difference source of isolation site thus in our study isolation source mastitis milk samples were examined, but in other research stool was the source. The difference in samples isolated from milk can be due to differences in geographical areas.