Effect of explants and different hormone Treatments on direct regenerationof potato Santé cultivar

This research that is a part of comprehensive study of tissue culture of potato to achieve appropriate hormone treatment for direct regeneration of Sante potato cultivar and to determine the best type of explant for direct regeneration has been conducted in 1389-90 for a year. at Islamic Azad University, Varamin Pishva Branch, faculty of agricultural. In this study thermotherapyed plant that emerged from meristem culture Sante cultivar, conveyed to mono bud planting for micropropagation. Then from these plants supplied leaf and internodal explant. Also healthy minitubers from this cultivar has been used to supply explant, that after sterilized conveyed to solid MS medium which supplemented with different concentrations of 2,4-D, Zeatin and one level of GA3 hormones. These concentrations of hormones included three levels of 2,4-D and Zeatin, hormones, and one level of GA3 Hormone. These plants was placed in light and 20-22 °C temperature to induce direct regeneration. Then after 28-3 days plantlet emerged on explants. Producing and colony of plantlets for use in agronomy was the ultimate purpose of this reaserch.