Isolation of campylobacter from poultry gizzards in urmia using PCR

Campylobacter species, especially Campylobacter jejuni is the most common cause of bacterial gastroenteritis around the world, often through the consumption of meat and poultry products, including chicken gizzard, are transmitted. In winter 1392 to spring 1393, This study has been aimed to determine the prevalence of Campylobacter species in poultry gizzard in Urmia, 80 samples of gizzard from the Protein Gostar Sina slaughterhouse located in the city of Urmia collected after culture, gram- negative and catalase- positive isolates selected, of the 16S rRNA gene was random and their General primers were amplified by PCR technique. The results showed that 62/5% of gizzards using selective culture of Campylobacter positive were detected. Diversity of Campylobacter isolates to the case: 40 percent Campylobacter jejuni, 20 percent Campylobacter coli and 40 percent Campylobacter lari detection in chicken gizzard was given. Contamination levels were also significantly higher in spring than in winter, which may be due to the high temperature of environment that was created the right conditions for Campylobacter. Therefore, sanitation and accurate monitoring of the destruction and packaging process for control of critical points and minimize the transmission of pathogenic bacteria such as Campylobacter Foodborne to meatand poultry products useful.