Identification of two commercial strains of Iranian and French rainbow trout (Oncorhynchus mykiss) using PCR-RFLP technique

In order to identify polymorphisms in the growth hormone gene for rainbow trout (Oncorhynchus mykiss), a total of 20 different samples were prepared from both Iranian and French populations and DNA extraction was performed using CTAB method. The polymerase chain reaction (PCR) was amplified fragment of 1825 bp the growth hormone gene. The amplified fragments were using enzymes including. Tag I, Hinf I, Mbo1, AluI, HinIII, HpaII and NdeI and fractionated on the 3% agarose gelelectrophoresis. In enzymatic digestion of the PCR products, Alu I and Tag I enzymes show polymorphism with a new pattern in 450 and 600 bp (by Alu I) and a new 500 bp (by TagI) which was different among Iranian and French populations. According to the present results, we could suggest that the enzymes AluI and TagI can be used as molecular markers for the identification of these two populations; and also be useful to distinguish between imported and native trout populations in aquaculture industry.