Cytogenetic study of khouzestanian water buffalo by G -banding method

In order to study of the khouzestanian water buffalo karyotype from whole blood of 200 Animals has been collected then 7ml of whole blood was cultured in RPMI1640 medium culture and phytohemagglutinin type A (PHA-A) was used as mitogen agent and incubated at 37ºC for 72 hours. Cell division was stopped in metaphase with adding 200 µl of colchicine to media after 72 hours. White cells has been driven by Sumner´s method¡ then chromosomes was spread on frizzed lams. Sumner´s methods were used for staining and banding of chromosomes. Cell spreads were fixed with heat and Entellan Merck. Centromere index and partial length of chromosomes were determined from photo micrographs with 200x enlargement. The results showed that all breeds have a diploid chromosome number of 2n=50. Each karyotype consisted of 24 pairs of autosomes ¡that 5 pairs of them were metacentric and 19 pairs of them were acrocentric. Chromosome number 1 with6.72±0.0178 partial length was the largest chromosome. X chromosome with6.25±0.056 partial length was the largest acrocentric chromosome and the Y chromosome with 1.872±0.016 was the smallest chromosome acrocentric.