Evaluation of Magnetic Silica Nanoparticles Treated with In-111 in SKBR-3 Cell Line
Message:
Abstract:
Introduction
Magnetic nanoparticles are as nanoscale materials causing a great revolution in the diagnosis and treatment methods in medical science. Suitable half-life of the In-111 radioisotope makes use for in vivo studies. In this study, in order to obtain optimal absorption and stability the first synthesis of the silicate magnetic nanoparticles (Fe3O4@SiO2) and stabilization of the In-111 radioisotope were investigated. Then, the permeability was examined in SKBR3 breast cancer cell line for using the diagnosis of diseases.
Materials and Methods
The magnetite core was prepared by precipitation method. Then it was used as the core for the synthesis of magnetic silicate nanoparticles. Nanoparticles were synthesized according to sol-gel method in the reverse micro emulsion using tetraetoxy silane (TEOS) and 3-amino propyl tree-ataxia silane (APTS) as the monomers and precursors. Then the In-111 radioisotope adsorbed on the surface of the magnetite silicate nanoparticles and formed the radio-conjugated. Finally, the entry of the radio-conjugated nanoparticles on SKBR-3, breast cancer cell line, is studied through the cell culture.
Findings: TEM results were shown the average size of the nanoparticles about 40 NM. The size is suitable for biological applications. The radio-analysis revealed more than 92 percent of the primary In-111stablized on the nanoparticles. The nanoparticles cell culture results are revealed the highest entrance efficiency about 26-27%- during the first hour from beginning the cultivation. The stability tests results revealed the stabilized radio-conjugated are stable during of washing and scattering and then considered as stable conjugation.
Discussion &
Conclusions
Due to the unique properties of In-111the mentioned nanoparticles, which are prepared using nano biotechnological methods would be able to apply for diagnosis purposes.
Language:
Persian
Published:
Ilam University of Medical Science, Volume:24 Issue: 1, 2016
Pages:
18 to 27
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