Isolation and Identification of Lactobacillus from breast milk by Molecular methods

Breast milk as the only source of nutrition in the first months after birth, an important factor in the development of microorganisms that are present in the intestines of infants. Given the importance of lactic acid bacteria, especially lactobacilli in human health, molecular recognition and classification of these bacteria could be an effective step in applying them in Functional products. The purpose of this research, detection and identification of Lactobacillus strains present in the breast milk. In this study, the strains in milk samples were isolated after enrichment in MRS broth, After microscopic examination, the basic characteristics of bacteria was determined by biochemical tests. Finally, to identify the molecular basis Identification of 16SrRNA sequences and genomic fingerprinting by REP-PCR was performed. Gram positive and catalase negative bacilli and cocci, were coded. Finally the PCR products were sequenced, and the sequences were analyzed with BLAST software. Sequencing results were analyzed using Ez Taxon server, the results showed that the strains isolated type of Pediococcus lolii, Lactobacillus rhamnosus and Lactobacillus fermentum. Breast milk can be considered as a source of lactic acid probiotic bacteria that may play a role in preventing of infant infectious disease have in the other hand the present study demonstrates the efficacy of REP-PCR genomic fingerprinting with marker (GTG) 5 in examining phylogenetic relationships and genetic variation in bacteria.