Study on association of leptin and growth hormone receptor genes polymorphisms with semen quality of Holstein bulls
Message:
Abstract:
Object and
Background
The purpose of this study was to evaluate the polymorphism of two candidate genes including Leptin (exon 2 and intron 2) and growth hormone receptor (promoter) genes and their effects on semen quality of Holstein bulls.
Materials And Methods
For this purpose information from two breeding stations including the Animal Breeding Center of Northwest (41bulls) and the National Animal Breeding Center and Promotion of Animal Products (28 bulls) were used. Information of 69 bulls, which were sampled between 2003-2013 years was used. Four qualitative sperm traits including volume of ejaculation (mL), sperm population (million/mL), and percentages of live sperm before and after freezing (%) were measured. Polymorphism of leptin gene was evaluated by PCR-RFLP and the variation in the growth hormone receptor gene was evaluated by PCR-SSR.
Results
The results of this study showed that all investigated loci were polymorphic and the frequency of TT, AT, AA genotypes in exon 2 were 0.28, 0.20 and 0.52, and frequency of T and A alleles, were 0.62±0.041 and 0.38±0.041, respectively. Additionally, genotype frequencies for AA, AB, and BB genotypes in the intron 2 were estimated 0.68, 0.32 and 0.16 and estimated A and B allele frequency were 0.84±0.031 and 0.16±0.031 respectively. For promoter of growth hormone receptor, results indicated that three genotypes of TG17/TG17, TG17/TG21 and TG21/TG21 had frequencies of 0.27, 0.48 and 0.25 and two alleles of TG21 and TG17 had frequencies of 0.49±0.042 and 0.51±0.042, respectively. There was Hardy-Weinberg equilibrium for the promoter of growth hormone receptor (P
Conclusion
Generally, there was a significant association (P
Language:
Persian
Published:
Journal of Ruminant Research, Volume:3 Issue: 3, 2015
Pages:
169 to 184
magiran.com/p1537498  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!