Evaluation of Bacillus and Lactobacillus strains isolated from Epinephelus coioides regarding enzyme activities

This study be done with purpose of comparison Bacillus and Lactobacillus strains isolated from the digestive tract of Epinephelus coioides regarding amount of enzyme activities. Thus aseptically, from different parts of the digestive system of E.coioides were sampled. After culturing in specific media and preforming biochemical tests and purification of different bacterial strains, specific activity of amylase, protease and lipase enzymes were measured by use of specific substrates in six strains of Bacillus genus and two strains of Lactobacillus genus. The findings indicate that specific activity of each of three enzymes in the strains of Bacillus genus is more than the strains of Lactobacillus genus. Regarding activity of protease, B9 strain had maximum activity with average 6.50 IU/mg protein which had significant difference (in level 0.05) with other strains. Respectively, maximum and minimum amylase activity observed inB9 strain of Bacillus (3/30 IU/mg protein) and L4 strain of Lactobacillus (0.43 IU/mg protein) which they had significant difference (in level 0.05), together. Also, maximum lipase activity observed in B9 (1.80 IU/mg protein) and B3 (1.66 IU/mg protein) strains of Bacillus which had significant difference (in level 0.05) with other strains.