Evaluation of inhibitory effect of Saccharomyces cerevisiae on B1 and B2 aflatoxins in culture media and Kilka fish meal

Aflatoxins are the most important metabolite of Aspergillus and some species of Penicillium. B1, B2, G1 and G2 are the main types of aflatoxins and the other metabolites are produced from these. In this study, inhibitory effect of Saccharomyces cerevisiae on B1 and B2 were examinated in two scales (culture media and Kilka fish meal). In culture media, were used from two concentration of B1 (12 and 16 ppb) and B2 (8 and 12 ppb) and two doses of the yeast (3 and 4%). In fish meal, were also two concentration of B1 (50 and 100 ppb) and B2 (25 and 50 ppb) and one dose of the yeast (4%). Changes of aflatoxins and the yeast growth were tested by HPLC and Spectrophotometer (OD: 600 λ respectively. Laboratory results were showed that B1 and B2 havebeen decreased to 90.6-92.8% and 89.9-94% respectively(for 3% Saccharomyces cerevisiae) and 93.3-94.6% and94.9- 95.8% respectively (for 4% Saccharomyces cerevisiae). In fish meal, B1 and B2 have been decreased to 85-90.79% and 87.05-87.07% respectively. The conclusion were showed that this yeast is able to significantly decrease of B1 and B2 aflatoxins in culture media and meal and can be used as biological tool in different feed particularly fish meal.