A survey on prevalence rate of Aflatoxigenic species of Aspergillus and residues of aflatoxins by ELISA method in Rainbow Trout feeds in Tehran and West Azarbayjan provinces - Iran

In this research 96 samples of Rainbow Trout fish feed, during the seasons of spring and summer of 2007 (every fifteenth of the month) were rondomized (by simple and stratified rondoms) to determine:1. The prevalence rate of aflatoxigenic species of Aspergillus in stored feed of cold-water cultural fish in West Azarbayjan cultural fish farms in both seasons (spring and summer);
2. The residues of total aflatoxin in stored feed of fish in cultural fish farms of West Azarbayjan in both seasons by ELISA method
3. The residues of that toxin in feed produced in aquatic feed factories in Tehran and West Azarbyjan provinces with the same method.
In order to study prevalence rate of toxigenic species of Aspergillus, standard No.997 of Iranian Standard Inistitute, were used. The produced colonies were examined microscopically. To determine the aflatoxins residues, ELISA method using Agra-Quant kit of Romer lab. company, were applied. The results of this survey indicated that only 8.3% of the samples were infected by A. flavus. A. parasiticus was not observed. There were no significant differences between the prevalence rate of AFT and seasons/monthes, either (P