Isolation and identification of Mycoplasma gallisepticum from commercial broiler Flocks in Ghaemshahr Town ship

Mycoplasmosis a common disease in the poultry industry. Among the most important illnessmaking in birds Mycoplasma, it can be called Mycoplasma gallisepticum. But so far, we did not have any research about finding Mycoplasma gallisepticum by using of PCR method in Ghaemshahr Town ship. The goal of this study (research) was isolation and identification Mycoplasma gallisepticum of Ghaemshahr Town ships commercial broiler flocks with using from microbiology and molecules (PCR) methods. In this research performed sampling from eighty one of Ghaemshahr Town ships commercial broiler flocks. at the beginning of this work , from commercial broiler flocks performed biopsy of windpipe, sirenx and airsocks and after transmitting samples to Razi institute, it began to culture PPLO bruth place and agar into sampling of tissues. in this research, from the whole eighty one sampled farm broiler, twenty farm broiler in regard to culture, and forty farm broiler in PCR experiment in regard to genus be positive as well as ten farm broiler in PCR experiment in regard to Mycoplasma gallisepticum species. The results indicate that Ghaemshahr Town ships farm broiler be contaminated to Mycoplasma gallisepticum and basic programme is necessary for control and prophylaxis disease s factor, and be recommended to making use of molecule s experiment such as PCR for identification diseases factor and immediate diagnosis.