Effect of eight weeks high-fat diet with endurance training on plasma levels of Amylin in male Wistar rats

Amylin is a polypeptide hormone prevents weight gain and obesity by loss of appetite. Therefore, the purpose of this study was the effect of eight weeks high-fat diet with endurance training on body weight and plasma levels of amylin in male wistar rats.
In this experimental study, 24 young male Wistar rats were divided into three groups (n=8) includes control (C), high fat diet training (ET᱐) and high fat diet (HFD) groups in age range 50 to 60 days and average weight 160±10 g. The (ET᱐) group rats run on treadmill without slope by 5 day/week, per session 20-63 min, 15-30 m/min velocity for eight weeks and they used a high fat diet. The control group used for initial sampling and high fat diet (HFD) group no train. High fat diet was included 60% fat, 24% CHO and 16% protein available two groups; C and ET. Blood sampling was conducted after latest training session with 12h fasting. The amylin concentration was measured by Cusabio Biotech elisa kit. The collected data were analyzed using the ANOVA and Tukey Post-Hoc tests (P There were no significant difference between amylin levels in (ET᱐) and (HFD) groups compared to control (C) group (p=0/25, p=0/867). The reduction of amylin concentration was no significant in (ET᱐) group than HFD group (p=0/543). The rat's body weight were higher significantly in ET᱐ and HFD than control group (p It seems that high fat diet for eight weeks with and without endurance exercise training does not change plasma level of amylin concentration in rats. However, high fat diet increase rats body weight and eight weeks endurance training was not able to reduce body weight in high fat diet-fed rats.