Survey of conformity between organoleptic and microbial culture techniques to diagnose cows mastitis and antibiogram test in milk of industrial and traditional herds

Study of management and hygienic factors involved in mastitis and also offering appropriate treatment is very effective in lactating animals production.
This study was to investigate the conformity between organoleptic and microbial culture techniques to diagnose mastitis in industrial and traditional dairy herds in Daland city and to determine the kind of pathogens in milk samples and also antibiogram test for the common antibiotics.
Eight dairy cattle herds (four as industrial and four as traditional) were selected from the region. Three cows from each herd that seemed to be infected to clinical mastitis (according to visual, tactile and etc.) were selected and two morning milk samples were collected from each. Initially, samples incubated in general culture to observe colonies and then, differential tests were performed to identify Staphylococcus aureus and Escherichia coli. Antiobiogram test was performed to determine the sensitivity to oxytetracycline, chlortetracycline and gentamycine.
Results of microbal culture showed the incidence of mastitis in the most of suspected cow. Staphylococcus aureus infection was observed in all industrial herd's samples except one, and in 100% of traditional herd's samples, while Escherichia coli infection was only observed in one-fourth of industrial herd's samples and in 100% of traditional ones showing a significant difference between them (P Results demonstrated the high conformity of organoleptic method with bacteriological method to diagnose clinical mastitis. Staphylococcus aureus in both industrial and traditional herds and also Escherichia coli mainly in traditional herds are mediated in dairy cows mastitis in this region. Udder pathogens in both kinds of herd in this region are relatively resistant to oxy- and chlortetracycline while gentamycine can be effective.