Bacterial Flora in Clean and Dust Polluted Air with FAME Technique. Ilam 2015

Air pollution by dust of the main problems in the country's West Frontier that is effective role on general health of residents. The presence of bacteria in the air and dust and their harmful effects on human health have been reported in various studies. This study is done to identify the bacteria natural air (free from dust) to dust polluted and compare both the number and diversity of their environment by FAME technique.
samples of natural air and dust contaminated with Particle deposition method on the plate or gravity and using sterile petri plates containing nutrient medium BHI Agar from 5 points in the city in 15 and 30 minutes was conducted in the summer. Plate transported to the laboratory and was incubated at 37 ° C for 24 to 48 hours. After the growth of bacterial colonies using biochemical tests and techniques FAME Bacterial colonies grown on the medium was studied and identified.
A total of 1114 bacterial colonies were counted on 30 plates collected from 5 locations . With Gram staining methods, MacConkey agar medium and Biochemical tests of 13 different bacteria were identified. In the dust air of the city of Ilam at 15 and 30 minutes most of the genus Rhodococcus (15 minutes) and Bacillus (30 minutes). From1114 colonies were counted; the number of 170 colonies of natural air and 944 colonies was dust in the air. The results showed that the highest number of bacteria has been gram-positive cocci in natural air and gram-positive bacilli in the dust air and was not observed pathogenic bacteria in natural air and dust.
The results showed that due to the high number of bacilli and spores bacteria in the air of dust are more resistant to adverse environmental conditions and that thereby the survival of most of them.