The effect of cadmium on viability and activity of some of the antioxidant enzymes in the callus of Catharanthus roseus

Catharantus-roseus is a medicinal and ornamental plant with growing attention toward its economical value. To determine cell viability based on trypan blue and MTT assay, cell suspension from callus of Catharantus-roseus were treated with different concentrations of cadmium-nitratee for 1, 3 and 6 days. Also cell suspension was used to investigate the cell morphology for a period of 3 days using 0, 20, 30 and 40 mM of cadmium nitrate. In addition, the callus was also treated for a same period and concentrations to estimate the level of lipid peroxidation and the activity of superoxide dismutase, catalase and guaiacol peroxidase. Data was analysed using one-way-ANOVA (Duncan-test) and pCatharantus roseus. Activity of the antioxidant enzymes increased significantly but was not able to compensate the caused damages.