Study of V617F mutation in JAK2 gene in patients of suspected thrombosis with Taq man Allele Specific Real Time PCR method
Author(s):
Abstract:
Aim and
With the aim of applying a method known as ''Taq man Allele- Specific Real-Time'' for diagnosis and screening of V617F mutation in JAK2 gene, this study was carried out in those patients being susceptible to thrombosis.
Background
Formation of the blood clot inside a blood vessel is known as thrombose or thrombosis. This complication usually appears through the blood platelets. The tendency to clot originates from external and genetic factors which in turn, is a result of changing in the clotting mechanism.With the aim of applying a method known as ''Taq man Allele- Specific Real-Time'' for diagnosis and screening of V617F mutation in JAK2 gene, this study was carried out in those patients being susceptible to thrombosis.
Materials And Methods
In this study, through referring to the Pathobiology Laboratory of RASHT city situated in Guilan province, 110 perfect bloods from the afflicted individuals and 105 perfect bloods from the ordinary individuals (lacking past record of the disease) were provided out of the referrers in order to study the thrombosis factors and, after DNA extraction from the whole samples, required measures to study presence of V617F mutation in JAK2 gene were taken by a method known as Taq man Allele-Specific Real-Time PCR, then, using statistical methods, the results were analyzed.Results
After confirming the accuracy of the desired technique applied on the control samples, the results of screening this method in the population of individual referring to study the factors of thrombosis, including factors of 2 and 5, presence of this change was suggested. Also, none of the V617F mutation in JAK2 was identified by screening on the samples of the control population.Conclusion
Our findings showed that the applied technique is sufficiently capable of diagnosing the V617F mutation in JAK2 gene. Also, lack of identification of V617F mutation in JAK2 gene in the population of those referring to the Pathobiology Laboratory for thrombosis in order to investigate factors of 2 and 5 showed that V617F mutation in JAK2 gene in the studied population is not propounded as one of the thrombosis factors. Keywords:
Language:
Persian
Published:
New Cellular & Molecular Biotechnology Journal, Volume:6 Issue: 22, 2016
Pages:
73 to 82
magiran.com/p1549695
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!