Sensitivity determination of Aspergilus Flavus to Itraconazol, Voriconazol and Caspofungin

Aim and Different studies have shown that despite the expanding number of antifungal agents, death rate caused by Aspergillus species has been increased during the recent decades due to drug-resistance occurrence, increased minimum inhibitory concentration (MIC) and cross-resistance among the isolated species. Regarding the lack of effective response to conventional treatments and antifungal susceptibility patterns of the most common isolated Aspergillus species.
Material and In this study, during 9 months; 20 clinical isolates and 20 environmental strains were studied. In the next stage, drug sensitivity test was done according to the NCCLS-M38P method. A fungal suspension was made with 1-4*104cfu/ml cellular range using 530nm spectrophotometer. Serial dilution of Itraconazol, Voriconazol (0.015-16mg/ml) and Caspofungin (0.007-8mg/ml) were performed and MIC was determined after 48Hr of incubation on 350c.
In studied strains we did not find any drug resistance gene. MIC range for Aspergillus flavus in Itraconazol was determined as 0.125-2mg/ml which MIC50= 0.5 and MIC90=1mg/ml. also for vericonazol range was 0.25-2mg/ml and MIC­­­50=0.5 and MIC90=1 determined and caspofungin 0.0131-0.125mg/ml and MIC50=0.063 and MIC50 = 0.063 and MIC90 = 0.125.
According to MIC range for Itraconazol, voriconazol and caspofungin , the studied aspergillus flavus strains were drug sensitive; also, we did not find any drug resistance strain.