Structure of Tumor and Localization of Na +/ K+- ATPase in Mouse (Balb/c nu), 4T1 Breast Tumor Model

Background and The 4T1 cell line is a laboratory model used in the study of tumors biology. This cell line is very tumorigenic with high metastatic capacity in different organs. In this study, histology and immunohistochemistry methods were used to investigate the structure and localization of Naﲯ ATPase enzyme in 4T1 cells induced breast cancer tumor in Balb/c nu mice.
The histological sections (4 µm) were stained using Hematoxilin-Eosin and IgGα5 special antibody was used for immunohistochemistry study.
Study of tumor structure showed abnormal proliferation and high mitogenicity in epithelial cells with high proliferation rate. Immunohistochemistry analysis revealed significant immunofluorescence, indicating abundant presence of NaﲯATPase enzymes (sodium potassium pump) in tumor cells.
Current results showed high rate of sodium potassium pump in plasma membrane of tumor cells. Immunofluorescence characteristic of 4T1 tumor cell lines make them appropriate candidate for antitumor studies.