Plasmid Profile Analysis of Aminoglycoside-Resistant Escherichia coli Isolated From Urinary Tract Infections

Uropathogenic E. coli (UPEC) is the primary cause of human urinary tract infections (UTIs) worldwide. Moreover, there has been renewed and growing interest in using older antibiotics for treatment, such as aminoglycosides.
The goal of this study was to determine the plasmid profile patterns of UPEC isolates harboring the aminoglycoside resistance gene aac(3)-IIa.
Patients and
A total of 276 uropathogenic E. coli (UPEC) samples were isolated from UTI patients at the Tehran heart center in Tehran, Iran. Antimicrobial susceptibility testing against five aminoglycosides was performed by the disk diffusion method, and the aac(3)-IIa gene was detected via PCR. Moreover, plasmid profiling was carried out on those UPEC isolates harboring the aac(3)-IIa gene. Finally, the similarities among these isolates were determined on the basis of their plasmid profiles.
The highest level of resistance was seen for tobramycin (24.6%), and the aac(3)-IIa gene was found in 51 isolates. Twenty-seven different plasmid profiles were identified among the isolates harboring the aac(3)-IIa gene, with the 15 kb plasmid being the most common. Moreover, no significant correlation was found between the resistance patterns and the number of plasmids. The cluster analysis based on the plasmid profiles grouped the isolates into five different clusters, of which cluster one was the largest (containing 14 of 51 isolates).
Our data suggest the monitoring of aminoglycoside resistance, and its consideration in the empirical therapy of UPEC infections.
International Journal of Enteric Pathogens, Volume:4 Issue: 2, May 2016
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!