The association between IL-27 gene polymorphism (-964 A/G) and clinical outcome due to infection with Helicobacter pylori

Helicobacter pylori is one of the most common pathogen bacteria in human being. Almost half of the population of the world are infected with this bacteria, and it has been known an important factor in gastrointestinal diseases such as; chronic gastritis, gastric ulcer and gastric cancer. Interleukin-27 is a pro-inflammatory cytokine expressed by a novel subset of CD4 Th cells, and it causes the occurrence and strengthening the inflammatory response. This study was aimed to analysis of association between IL-27 polymorphisms and clinical outcomes due to infection with H. pylori.
A case-control study has been performed on 434 people 149 patient cases (100 gastritis and 49 ulcerative gastritis), 58 gastritis cases but H. pylori negative and 227 healthy controls. Genomic DNA was extracted and genotypes of IL-27 (-964 A/G) polymorphism were assessed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Finally the results were compared between these patient and control groups.
The frequency of A allele was higher in gastric patients (78.5%) in comparison with the control group (75.6%). However, these differences were not significant. In addition, the distributions of genotypes were not significantly different between the study groups.
These results suggest that IL-27 (rs964 A/G) polymorphism gene is not directly involved as a genetic risk factor in the predisposition to H. pylori.