The Cytotoxic Effect of Vitex pseudo negundo Fruit in Breast Cancer (MCF7) Cell Line

Breast cancer is the most common cancer affecting women so that it is the second common cancer (after lung cancer) in women. Vitex pseudo negundo is used as a traditional medicine. Recently, the biological activities of Vitex pseudo negundo plants have been reported as possessing anticancer, antibacterial, antiulcer and antifungal properties. However, the antitumor effects of this medicine have not been studied in cancer cell lines. The aim of this study was to investigate the antitumor effect of Vitex pseudo negundo fruit on breast cancer cell lines.
Breast cancer (MCF7) cell lines were cultured in DMEM medium. Vitex pseudo negundo fruit was extracted; and different dilutions of Vitex pseudo negundo extract (5mg/mL to 100mg/mL) were added to cell culture. Cell viability was quantitated by MTT assay after 72 hours.
The findings indicate that the extract of Vitex pseudo negundo fruit on MCF7 cancer cell lines had cytotoxicity in all concentrations and the highest inhibition was 50 and 100 mg/ml concentrations.
Our study shows that Vitex pseudo negundo fruit extract has cytotoxic effects on tumor cells, It seems that Vitex pseudo negundo fruit could be considered as a promising chemotherapeutic agent in cancer treatment.