Effect of olive leaf extract on rooster semen storage

Antioxidant effect of olive leaf extract (OLE) was studied on motility, viability, plasma membrane integrity of spermatozoa and malondialdehyde production in 12 Ross 308 roosters at their 30 weeks of age. Semen samples were collected by abdominal massage in 5 times. In each session after the initial sperm assessment, collected samples were pooled and diluted with Sexton extender.Samples were split into five parts and the concentrations of 0 (control), 50, 100, 150 and 200 µg/mL OLE were added to each part, then,the samples were incubated for 72 hours at 4 degree Celsius. Progressive motility, viability and plasma membrane integrity were evaluated at 0, 24, 48 and 72 hours of storage and production of malondialdehyde were analyzed after 48 hours of storage. Adding 100 µg/mL OLE to semen reduced malondialdehyde production (P