Study of the Repair of Bone Defect using Chitosan - Gelatin Membrane and Bone Marrow Stromal Cells in Male Albino Wistar Adult Rat

Aim: This study aimed to evaluate the histomorphometric and immunohistochemical parameters of the repair of femoral bone defect using bone marrow stromal cells on gelatin – chitosan membrane in adult Albino Wistar rats.
In this experimental study, sixty male Albino wistar adult rats were equally divided into five groups as follows: Control group that received no treatment after bone defect. Sham group that after bone defect, the culture medium was injected locally at the site of defect. Gelatin-chitosan group that membrane was used into bone defect. Cell group that nonautolog BMSCs were injected locally into defect .Cell-GC group that cell transplantation with chitosan - gelatin membrane were used into the bone defect.
The mean area of trabeculae in groups of membranes and cells significantly increased when compared to the control group.The mean number of osteocytes and cells in the bone defect in cell group significantly increased when compared to the control group.No significant difference were found in chitosan - gelatin and sham groups compared to control group but the mean number of osteocytes significantly decreased in BMSCs with gelatin-chitosan scaffold group compared to control group (P BMSC transplantation and gelatin-chitosan scaffold are effective in repair of bone defect. However, the use of BMSCs with gelatin-chitosan scaffold is not effective in repair of femoral bone defect.