Effect of plant growth regulators on direct Shoot regeneration of Henbane

In order to study, in vitro bud induction and shoot regeneration of different explants (shoot tip, axillary bud, hypocotyl and cotyledon) of Henbane on MS medium enriched with various concentrations of Kinetin (0, 1, 3 and 5 mg.l-1) alone or in combination with Indole-3 acetic acid (0, 0.1 and 0.5 mg.l-1) this research was investigated in Urom Zist Tak knowledge-based company plant tissue culture lab at 2013. ANOVA results revealed that the maximum buds inducted (average of 41.62 buds per explants) were in 5 mg.l-1 Kin and 0.5 mg.l-1 IAA , the maximum shoots regenerated (average of 155.67 shoots per treatment) were in 5 mg.l-1 Kin and 0.5 mg.l-1 IAA in shoot tip explant and minimum bud induction (average of 1.62 buds per explant) were in 5 mg.l-1 Kin and 0.5 mg.l-1 IAA , the maximum shoots regenerated (average of 5.7 and 6.5 shoots per treatment) were in MS medium without Kin in hypocotyl explant. The regenerated shoots were rooted in MS and ½ MS media fortified with different concentration of IAA and IBA (0, 1.1 and 2.2 µM) at four weeks. The maximum average root induction (87.50 roots) were MS medium treatment of 1.1, 2.2 µM IBA. The successfully acclimatized rooted plantlets were transferred to green house after 3 weeks with 90 percent of survival rate.