Gelatin-chitosan coating enhances L929 fibroblasts proliferation on supramolecular nano-fibrous scaffolds

Aim: The goal of tissue engineering is regeneration and restoration of damaged tissues and organs. Scaffolds are the main part of tissue engineering ¡and gelatin/chitosan surface modification is one of the proper methods for surface modification of polymer scaffolds. Evaluation of fibroblasts behavior on gelatin/chitosan coated supramolecular PCL (SP-PCL) was the aim of this study.
SP-PCL nano-fibers were prepared by electrospinning. Morphology of the nano-fibrous mats was investigated using scanning electron microscopy MTT assay was performed to examine cell activity on the nano-fibers. DAPI staining was carried out to evaluate cell viability.
The SEM images of the cells onto the scaffold showed good biocompatibility of the scaffold. In addition, the MTT assay results showed a proper metabolic activity of cells on the scaffolds The sPCL nano-fibrous mats coated with gelatin-chitosan blend show significantly (p The experiments showed that the nano-fibrous electrospun SP-PCL coated with gelatin/chitosan blend, provides a suitable environment for fibroblasts adhesion, and proliferation, possibly because of similarity to nano-fibrous natural ECM network.