Comparison of Dentin Microshear Bond Strength of Chlorhexidine on Two Step Self-etch Adhesives with and without Fluoride after 6-month storage in water

Regarding the possible leakage of the bacteria in the cavity; the use of antibacterial agents such as chlorhexidine in the adhesive restorations has been proposed. However, the effect of this process on the long-term bond strength to the dentin has not been studied completely. This study assessed the effects of chlorhexidine on the dentine micro-shear bond strength of two-stage self-etch adhesives with and without fluoride after 6-month storage in water.
Subjects and

In this in vitro study, 40 extracted human mandibular third molars were obtained and after dentin exposure and polishing with silicone carbide for standard smear layer preparation; they were assigned into 4 groups. With or without using chlorhexidine adhesives of Clearfil SE Bond and Clearfil Liner Bond F were applied to the dentin surface and the teeth were restored with Z250 composite. The teeth were incubated for 6 months at 37 °C temperature and 100% moisture and received 1000 thermal cycles. The microshear bond strength of the specimens was determined and their fracture patterns were determined by streomicroscope. The data were analyzed by one-sided analysis of variances test while the paired comparisons were done by Tukey test.

Application of chlorhexidine at dentin surface since performing by adhesive sel-fetch did not cause any seriouse adverse effects on bond strength. Mean±standard deviation of the bond strength values with and without the application of chlorhexidine for the Clearfil SE Bond were 20.66±3.27 and17.48±7.11 MPa. The figures were 31.63±7.33 and 31.42±11.39 MPa for Clearfil Liner Bond F adhesive (P>0/05).

Because of antibaterial effect of chlorhexidine and having no negative effect on bond strength; surface preparation with this agent beside using fluoride-containing adhesives is suggested to eradicate the existing bacteria and decreases microleakage in the clinical condition without compromising bond strerngth. ►Please cite this paper as: Zakavi F, Mombeyni M, AlikhaniA, Karimi S. Comparison of Dentin Microshear Bond Strength of Chlorhexidine on Two Step Self-etch Adhesives with and without Fluoride after 6-month storage in water.