Study on the effect of plant growth regulations (PGRs) on micropropagation of gavane-gaz-angubin (Astragalus adscendens Boiss & Haussk) via apical meristem

Gavane-gaz-angubin (Persian Manna) belongs to Astragalus genus (Leguminosae). This plant is one of the most important species in Iran for its medicinal value. Considering the low propagation rate of this plant from seed and cutting, the feasibility of in-vitro propagation of this plant was investigated. Effect of different types and concentrations of cytokinins such as BAP, KIN, and Ze (0.5, 1, 2 and 4 mg/l) in combination with NAA (0, 0.5 mg/l) were evaluated for production of apical bud in MS medium. Experiments were conducted in a factorial based on completely randomized design. Shoots were transferred to rooting medium and this medium contained different concentrations (0, 0.3, 0.6, 0.9 mg/l) of IBA or NAA. Results showed that not only the significant effect of different types and concentrations of cytokinins, but also confirmed effect of various concentrations of NAA on shoots number, leaf number and shoots length. Results indicated that MS medium containing 4 mg/l BAP lead to production of highest shoot number (8.5 shoots per explants) as well as the highest leaf number. The highest rooting production (100%) obtained in MS medium with 0.3 mg/l NAA, Likewise. More than 40 % of plants were adapted in pots with the mixture of soil, peat and perlite (1:1:1, v/v).