Standardization if the tetrazolium test in East Beech

To perform any breeding work on seed, determination of its life ability amount via germination test is very important. In general, biotechnical tests present rapid methods for evaluating viability of seed samples and specificalty to assess non-germinable seeds which seems to be dormant through the measurement of weak or dead tissue. Tetrazolium test is the most common of these tests. This study aims to optimize Tetrazolium test conditions including concentration, temperature and storage time on seeds in Fagus orientalis. Factorial experiment was conducted in completely randomized design in three replications. the results of this experiment was compared with the obtained results from embryo culture test (in MS medium) for evaluating the best condition of tetrazolium. Soaking treatment of seeds for 18 h at 30°C in a solution of 0/5% Tetrazolium test results with the greatest similarity to embryos cultured was selected as suitable treatment for germination test using tetrazolium protocol in Fagus orientalis.