Effect of Feeding Oxidized Soybean Oil with Pomegranate Seed on the Blood Antioxidant Capacity, Enzyme Activity and Inflammatory Factors of Periparturient Saanen Goats

This experiment was carried out to study the effect of oxidized soybean oil (OSO) against antioxidative ability of Pomegranate seeds (PS) on body antioxidant status and oxidative stress complications in periparturient Saanen goats. Blood total antioxidant capacity (TAC), Malondialdehyde (MDA) concentration, C-reactive protein (CRP), rheumatoid factor (RF) & enzyme activities were investigated three weeks pre and post-partum, through a completely randomized design with repeated measurements. Dietary treatments (T) included base diet and 4% fresh soybean oil (FSO) (T1), 4% OSO (T2), 4% OSO and 8% milled PS (T3)(DM basis). Three days after parturition similar diet was fed to all groups, to evaluation of durability of treatment effect. The blood enzyme activities of alkaline phosphatase (ALP), creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT) & lactate dehydrogenase (LDH), CRP, RF, MDA and TAC had ascending pattern in T2 vs. T1 and descending trend in T3 vs. T2. The ascending pattern in CRP, ALT, MDA and ALP and the descending trend in RF, TAS, CRP and ALP were significant statistically. Also two weeks after feeding similar diet, all parameters except CK, RF, TAC & MDA changed likewise previous pattern. Generally, the possible roles of lipid peroxidation derivatives of OSO led to progressive oxidative stress complications and reduced Blood TAC vs. FSO. Nevertheless, PS antioxidative components had beneficial effects on blood TAC and lowering role on oxidative stress signs.