Expression of CD86 Co-stimulatory gene in colon polyps
Abstract:
Introduction
Since the majority of colorectal cancers originate from polyps in the area, therefore, the investigation of the immunological gene expression in colon polyps can be used as a factor for the detection of genetic and pathological differences in polyps. The aim of this study was investigating the expression of CD86 gene in colorectal polyps and its association with malignancy.
Materials And Methods
In a descriptive-analytical study during (2012 to 2014), forty-nine biopsy samples were collected during colonoscopy from the patients with colorectal polyps and ten healthy subjects for normalization. A questionnaire including clinical and demographic data was filled out for all cases. Mucosal mRNA expression of CD86 gene was investigated using Real-Time PCR and Fold Change of gene expression (2-DDCt) method.
Results
A total of 49 patients including 27(55.2%) male and 22(44.8%) female with mean age of 53±15.3 were evaluated in this study. Adenoma and hyperplastic polyps were reported in 69.3% and 30.7% patients respectively. More than 90% of adenomatous polyps were spread out to the colon while the others were located in the rectum. Based on Relative Quantitation method, over-expression of CD86 genes in colon polyps were observed (Polyp ≥2-fold normal) and down-expression of CD86 in rectum polyps were noticed in comparison with normal samples (Polyp≤0.5-fold normal).
Conclusion
Comparing mRNA expression level of CD86 gene in colon polyps with the profile of mRNA expression of polyps in the rectum indicates the presence of distinct molecular and immunological mRNA expression between polyps of these two sites.
Language:
Persian
Published:
Pages:
13 to 24
magiran.com/p1587847  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!