The effect of different concentrations of kinetin and naphthalene acetic acid on micropropagation of Lisianthus (Eustoma grandiflorum)

One of the most important and applicable tissue culture techniques is micropropagation. Lisianthus (Eustoma grandiflorum) is an ornamental plant which studies on its micropropagation is insufficient. The aim of this study was to evaluate the effect of different concentrations of NAA and KIN on micropropagation of lisianthus. For this purpose, the explants were cultured on MS medium with different concentrations of NAA and KIN (0, 0.5, 1 and 2 mg l-1). Results showed that the longest shoot (2.325 cm/explant) was obtained in medium containing 1 mg l-1 KIN (without NAA). The largest number of shoots (with average of 2.800 and 2.550/explant) was produced in medium containing 1 and 0.5 mg l-1 KIN (without NAA). The highest number of node (with average of 8.358/explant) was obtained in medium supplemented with 0.5 mg l-1 (without NAA). Maximum number of roots (with average of 2.650/explant) was produced in medium containing 0.5 mg l-1 NAA along with 2 mg l-1 KIN. Regenerated plants showed 100% survival in ex vitro conditions.