Intraspecific study of Carpinus orientalis populations using seed storage protein pattern

Ecotourism and ecological and conservational importance of Hyrcanian forests are among the most important reasons for studying arboreal species of Hyrcanian forests. The main purpose of this study is to explore genetic diversity of
Carpinus orientalis in the study area; north of Iran, by using of morphological and SDS-PAGE of the seed storage proteins patterns. Seed samples were collected from different populations of provinces, Gilan, Mazandaran, Golestan and Khorassan-e-Shomali. The morphological studies performed by 28 characteristics analysis. Seed proteins extracted by Tris-Glycine buffer and then total protein quantified by Bradford assay and protein pattern of the populations was determined by SDS-PAGE technique. Morphological and bands electrophoresis patterns data were analyzed by PAST software. Results showed that, SDS-PAGE electrophoresis pattern of populations have 10 band polymorphisms. The maximum numbers of bands are related to Chalus and Kiasar populations, in the Mazandaran, and minimum numbers of bands are related to Chichal populations, in the Guilan. Resulted clusters from morphological and seed storage protein were different forms. Furthermore, the speed of morphological and genetic changes are not comparable.