Investigation of genetic polymorphism in leptin receptor and Melanocortin 4 receptor genes in native cattle of Guilan province (Bos indicus)

The experiment was concluded to study the genetic polymorphisms for Exon 2 leptinreceptor (LEPR) and Exon 1Melanocortin4 receptor (MC4R) genes in native cattle in Guilan province. In order to blood samples were collected randomly from 96 native cattle in Guilan province. After extracting DNA by salting out method, PCR-SSCP was used to amplify a fragment of 261 bp from exon 2 LEPR gene and a fragment of 213 bp from exon 1 MC4R gene. In the sample of study, three patterns AA, AB and AC for LEPR gene and six patterns AA, AC, AD, AE, AF and AG for MC4R gene were observed. Frequencies of genotype were 0.062, 0.615 and 0.323 for LEPR gene and 0.074, 0.21, 0.253, 0.158, 0.105, 0.105 and 0.095 for MC4R gene respectively. Population genetic analysis showed that LEPR and MC4R genes were out of Hardy-Weinberg equilibrium but both genes were highly polymorphic and had great genetic diversity.