Genetic Diversity Assessment of Lines and Varieties in Winter Rapeseed (Brassica napus L.) using RAPD and SSR Molecular Markers

The eight SSR′s and 15 RAPD′s primers were used to investigate of genetic diversity and relationship between 20 winter rapeseed genotype. Totally 164 bands were detected from Which 154 were polymorphic. The RAPD primers namely Oligo-670 with 16 bands showed the highest number of detected bands. The mount of Nei′s¡ Shanon and PIC index for Na12-B02¡ Ni4-B03¡ OL10-G04¡ Oligo-543 and Oligo-670 indicated that theses SSR and RAPD markers could be used to assessment of genetic variation. The result of cluster analysis using UPGMA algorithms clustered 20 studied genotype in 5 distinct groups with 2¡ 7¡ 5¡ 4 and 2 genotype in respected group. Discriminant function analysis using the Fisher′s linear method showed that the UPGMA method separated the genotypes with 70 percent accuracy.