Effects of Plant Growth Regulators on Callus Induction via Shoot Bud Meristems of Single Branch NazCultivar of Sesame (Sesamumindicum L.)

Sesame (Sesamumindicum L.) as an ancient plant has traditionally used for centuries as a medicinal plant due to its natural antioxidants and plenty of medicinal properties. Seeds from different cultivars of sesame have various nutritional values and different genotypes showing different response to mature shoot buds meristems. In present experiment callus induction and plant regeneration of sesame cultivar (Single Branch Naz) have been considered using different hormonal combinations in a factorial experiment based on completely randomized design with three replicates. In order to evaluate callus induction¡ 2¡4-D in three levels and NAA in four levels were used as auxinsources. In current study¡ percentage¡ fresh weight and diameter of induced callus were compared. Results in different environments showed that the highest percentage of callus was obtained using 0.4 mgl-1 2¡4-D hormone. Also¡ in terms of fresh callus weight maximum result was belong to combination of 0.2 mgl-1 2¡4-D and 0.16 mgl-1NAA hormones. Furthermore¡ in callus diameters the highest amount was allocated to combination 0.2 mgl-1 2¡4-D and 0.12 mgl-1 hormones. However¡ kinetin played a significant role in increasing the percentage of regeneration¡ so that. The best response was belonged to combination 2 mgl-1 Kin and 0.4 mgl-1 NAA hormones.