Evaluation of Corrosion Behavior, Bioactivity and Cytotoxicity of Nanostructured Hardystonite Coating on Ti-6Al-4V Substrate

In this study, Hardystonite powders were coated on Ti-6Al-4V alloy using electrophoretic deposition (EPD). Hardystonite was made by sol-gel technique. Structure, morphology and elemental analysis of produced Hardystonite were investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). Cytotoxicity of Hardystonite nanoparticles was investigated (MTT assay). Nano Hardystonite powder was electrophoretically deposited on titanium substrates. The coated samples were observed by SEM. In order to investigate bioactivity, coated substrates were immersed in simulated body fluid (SBF) for 7, 14 and 21 days. Scanning electron microscope (SEM) was used to study structure and morphology of layer before and after immersing in simulated body fluid (SBF). Tafel polarization test was used to evaluate the corrosion and protection properties of coatings. The bioactivity of the coating was confirmed using immersion test. The tafel results showed that the corrosion resistance of substrate increases by Hardystonite coating. The results of MTT assay confirmed the cell compatibility of hardystonite nanoparticles. The obtained results of this research showed that Hardystonite can be coated on Ti substrate using electrophoretic deposition. Also, corrosion behavior and biological properties of Ti substrate is improved and Hardystonite Coating can be used as a novel bioceramic coating.