Investigation on destructive effect of water-born CuO nanoparticles on gill histopathology of rainbow trout, Oncorhynchus mykiss

Nanotechnology as a modern science is rapidly expanding which can influx many biological aspects due to large extent. For this reason, concerns about the risks and consequences of this technology are rising in environment and especially in water resources. The purpose of this study is to investigate the effect of waterborn Cuo Nanoparticles on Oncorhynchus mykiss gill tissue, to detect a suitable biomarker for noticing Copper in Water Resources. Samples weighted 18±3 gr and exposure to Cuo Nanoparticels for a week with 25, 30, 50 and 100 ml/g doses. Physicochemical properties of water were 14±2 Cº temperature, 8-8.5 pH and 270 mg/l Caco3. At the end of 7 days, 9 fish were catch randomly from each treatment and second gill from left side of fish were removed for histological review. Hyperplasia, Oedema, Epithelial lifting in secondary lamella, lamellar aneurysm and Leukocyte infiltration was observed in gill tissues. In addition, the damage was determined at various concentrations of copper nanoparticles. Result showed that Cuo Nanoparticles, as copper ions, have significant adverse effects on gill tissues of O. mykiss and gills can be a suitable biomarker for copper in water resources. However, comparison of the extent and severity of injury stated that copper nanoparticles cause much less injury than copper ions.