Fraud Identification of Cow's Milk in Buffalo's Milk and It's Products Using the Polymerase Chain Reaction
Author(s):
Abstract:
Background
In the dairy industry, protection against species substitution or admixture is important for several reasons, including frequent human adverse reactions toward some species milk proteins, and trade and government regulations.Objectives
The objective of the present study was to assess the purity of buffalo milk and its products offered as pure buffalo in the market.Methods
Using species-specific primers, a duplex polymerase chain reaction (PCR) assay was performed to detect the fraudulent addition of cows milk to buffalos milk and its products.Results
The limit of detection of cows milk in buffalos milk, yogurt and cheese is 1, 2 and 4%, respectively. Undeclared presence of cows milk was detected in 70% of the milk samples, 64% of the yogurt and 52% of the cheese samples. In 10% of the yogurt samples and 14% of the cheese samples, no apparent buffalo-related amplification product was observed, suggesting that cows milk was entirely substituted for buffalos milk in these samples.Conclusions
To avoid unfair competition and to assure consumers of accurate labeling, using this method is recommended for regulatory agencies.Keywords:
Milk , Cow , Buffalo , Adulteration , Species Identification , PCR
Language:
English
Published:
Jundishapur Journal of Health Sciences, Volume:8 Issue: 4, Oct 2016
Page:
4
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