Mutagenesis in rice to develop drought tolerance and assessment of genetic variability of mutants using ISSR marker
Abstract:
In this research, EMS mutagen was applied to create genetic variability in rice (cv. Neda) and its mutagenesis effect was investigated in drought tolerance improvement. M2 mutants were evaluated at germinating and seedling stages under osmotic stress of PEG (-0.8 MPa) which resulted to isolating 9 drought tolerant lines. The lines along with their original cultivar also were evaluated in field under restricted irrigation regime which two mutants viz. MT90 and MT149 based on stress tolerance index (STI) were identified as drought tolerant lines. ISSR markers were used to assess genetic variability induced by mutation in mutant lines, 69.4 percent of which detected polymorphism between original cultivar and mutant lines. Mutation-induced genetic variation was estimated equal to 23.5 percent in the studied genotypes and cluster analysis using UPGMA method differentiated them into three main classes which mutants-containing groups produced higher yield and STI relative to original cultivar group under drought stress. Based on the results, MT149 mutant can be introduced as a drought tolerant line at both seedling and whole plant stages. It can be concluded that EMS-induced mutation had a desire effect in developing drought tolerant mutants and ISSR markers can be applied for differentiating drought tolerant mutants in rice mutation derived populations.
Language:
Persian
Published:
Journal of Molecular and Cellular Research, Volume:29 Issue: 2, 2016
Pages:
186 to 199
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