Effect of different concentrations of kinetin and putrescine on potato microtuberization (cv. Agria)

This experiment was conducted in order to investigation the effect of different concentrations of kin (0, 1 and 2 mg.l-1) and Putrescine (0, 20, 40, 80 and 160 mg.l-1) with 8% sucrose and 8% agar on the microtuberization of potato. This study was conducted at the Plant Tissue Culture Laboratory, Department of Horticultural Sciences, University of Tabriz. This study was conducted as a factorial experiment in a completely randomized design with three replications. Results of data analysis showed that none of the traits except percent microtubers non dormant in the second month, and shoot weight were not affected by different concentrations putrescine. Percent of non dormant microtubers in the second month increase on concentration of 20 mg.l-1 and increase shoot weight was 80 mg.l-1 putrescine. However, the effect of different concentrations kinetin was significant in relation to the creation microtuberization. In this experiment, the best concentration of kinetin for most traits microtuberization 2 mg.l-1. Also the best of putrescine concentration for weight microtuberization in media containing 2 mg.l-1 kinetin, 40 mg.l-1, which increase microtuber weight was 0.16 mg.l-1