Isolation and characterization of diesel-oil degrading bacteria from petroleum contaminated soil at Khozestan provenance

Diesel oil is the major product of crude oil. This product is the main pollutant of environment and has hazardous effects on the environment. Bioremediation is the best method for remediation of this pollutant, because this method use nature potential for remediation and also this method has a low cost.
In this study, for isolation of diesel-oil degrading bacteria sampling performed from contaminated regions. The prevalent diesel-oil degrading bacteria were identified by biochemical and molecular methods. Some properties in these bacteria were analyzed such as: hydrophobicity and emulsification activity. The percentage of diesel-oil degradation was assayed for each strain by Gas Chromatography (GC) and qualitative test.
In this research 34 diesel-oil degrading bacteria were isolated. From these strains 10 strains were selected according to diesel-oil degradation. These strains belong to Achromobacter¡ Enterobacter and Klebsiella. The half percentages of diesel-oil were removed by these strains in 10 days of incubation. The best strain for degradation of diesel was Enterobacter cloacae strain L2 (72%). The highest hydrophobicity is related toKlebsiella oxytoca strain K2 (76%) and the best emulsification activity belongs to Achromobacter denitrificans strain B2 (58 %).
Discussion and The results of this research confirmed that diesel degrading bacteria have sufficient diversity in soil contaminated in Iran. These bacteria have good potential for remediation of diesel pollution from soil.