Protective Effects of Melissa officinalis (Lemon Balm) on Sperm Parameters and Spermatogenesis Quality in Rats Exposed to Lead

Some medicinal plants with their antioxidant properties have protective effects on sperm parameters and neutralize the harmful effects of lead exposure..
The present study aimed to determine the effects of Melissa on sperm parameters and spermatogenesis quality in adult rats exposed to lead..
This animal experimental study was conducted on 45 male Wistar rats weighing 180 ± 20 g, which were allocated randomly into five groups (n=9): distilled water gavages (control) (0.5 ml); intraperitoneal distilled water (i.p. control) (0.5 ml); Melissa (100 mg/kg/bw); lead acetate (10 mg/kg/bw); and lead acetate (10 mg/kg/bw) plus Melissa (100 mg/kg/bw). After a 30-day treatment, the animals were sacrificed. Sperm parameters (count, motility, morphology, and chromatins assay) and weighing of the epididymis and testis were performed..
The results showed that in the lead acetate plus Melissa group, epididymis weight (0.37gr vs. 0.32gr), testis weight (1.40 vs. 1.28g ), sperm motility (4.44% vs. 33.2 %(, and viable sperm (90 vs. 50) were higher than the lead group. However, testicular weight, normal morphology, and DNA percentage showed insignificant differences among the study groups (P > 0.05)..
The results of the present study indicate the protective effects of Melissa officinalis on some sperm parameters and spermatogenesis quality in rats exposed to lead..