Prevalence of CTX-M-15 type of beta-lactamase gene in Escherichia coli strains using PCR method

Beta-lactamase enzymes are the most important resistance factors among Gram-negative bacteria to the beta-lactam group of antibiotics. This study was conducted to determine the prevalence of extended-spectrum beta-lactamases (ESBL) in Escherichia coli isolates using PCR method.
This descriptive – analytic study was conducted on 120 Escherichia coli samples isolated in hospitals in Sari in northern Iran during 2013. Antibiogram was conducted using combined disk method to determine the sample resistance. The presence of β- lactamase gene of CTX-M-15 in ESBL was assessed using PCR method.
Out of 120 Escherichia coli, 98 (81.6%), 15 (12.5%) and 7 (5.8%) bacteria isolated from urinary tract, blood and wound, respectively. Multiple drug resistance were seen in 98% of urine samples, 12.7% of blood samples and 3.6% of wound samples (P Beta-lactamase enzymes were detected in high percent of Escherichia coli isolated from urine samples.